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An efficient and reproducible method for regeneration of

Jul 01, 2011  An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201 - ScienceDirect New Biotechnology Volume 28, Issue 4, July 2011, Pages 418-422 Research paper

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An efficient and reproducible Agrobacterium-mediated

Oct 26, 2019  In this present study we report a reproducible Agrobacterium -mediated transformation system for the spring wheat Fielder that yields transformation efficiencies up to 25%. This system has been widely used to introduce genes of interest as well as for CRISPR/Cas9 based genome editing [ 15 ]. Materials and methods Plant material

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An efficient and reproducible method for regeneration of

An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201 Abstract Tissue culture is one of the tools necessary for genetic engineering and many other breeding programs.

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Correction to: An efficient and reproducible ... - Plant

Dec 17, 2019  In the Materials and methods section, under the subheading “Immature embryos isolation”, the term 0.44 mg L −1 should read as 0.44 g L −1. Under the subheading, “Resting period, callus induction and selection of transformed material” the term 5 mg L −1 and 15 mg mL −1 should read as 5 g L −1 and 15 mg L −1.

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(PDF) An efficient and reproducible method for

Feb 01, 2011  An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201 Authors: Shabir H Wani Sher-e-Kashmir...

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An efficient and reproducible method for regeneration of whole

Jul 01, 2011  An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201. Author links open overlay panel Shabir H. Wani 1 4 Gulzar S. Sanghera

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An efficient and reproducible method for development of

Apr 03, 2017  高达10%返现  An efficient and reproducible method for development of androgenic haploid plants from in vitro anther cultures of Camellia assamica ssp. assamica (Masters) Vijay Kumar Mishra , Ruchira Bajpai Rakhi Chaturvedi In Vitro Cellular Developmental Biology - Plant 53 , 239–248 ( 2017) Cite this article 542 Accesses 7 Citations

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Correction to: An efficient and reproducible

Correction to: Plant Methods (2019) 15:121 10.1186/s13007-019-0503-z. In the original publication of the article [], a few errors were identified.In the Materials and methods section, under the subheading “Immature embryos isolation”, the term 0.44 mg L −1 should read as 0.44 g L −1.. Under the subheading, “Resting period, callus induction and selection of transformed

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CiteSeerX — AN EFFICIENT AND REPRODUCIBLE

Among the single treatment, BA at an optimal concentration of 7.5 µM was most effective in inducing multiple shoots where 90% explants responded with an average shoot number (9.6 ± 0.3) and shoot length (1.7 ± 0.1 cm) after 6 weeks of culture.

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(PDF) An efficient and reproducible Agrobacterium

Oct 26, 2019  An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.) October 2019; ... Plant Methods. 2005;1(1):5. 15.

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An efficient and reproducible method for regeneration of whole

An efficient and reproducible method for regeneration of whole RESEARCH PAPER An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201 Shabir H. Wani1,4,, Gulzar S. Sanghera2 and Satbir S. Gosal3 1 Q2 2 Department of Plant Breeding and Genetics, Punjab Agricultural

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An efficient and reproducible method for regeneration of whole plants

An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201 Author: Shabir H. Wani, Gulzar S. Sanghera, Satbir S. Gosal Source: New biotechnology

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An efficient and reproducible method for in vitro propagation

Jan 01, 2013  Request PDF An efficient and reproducible method for in vitro propagation of Cassia Alata L. - an important woody medicinal plant A rapid and efficient protocol for the large-scale propagation ...

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IJPBS Article- AN EFFICIENT AND REPRODUCIBLE METHOD FOR

AN EFFICIENT AND REPRODUCIBLE METHOD FOR IN VITRO PROPAGATION OF CASSIA ALATA L. – AN IMPORTANT WOODY MEDICINAL PLANT MD RAFIQUE AHMED, MOHAMMAD ANIS AND IM AREF: DOI: Abstract: A rapid and efficient protocol for the large-scale propagation of an important woody medicinal plant, Cassia alata L., is described.

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An efficient and reproducible Agrobacterium-mediated

Nov 11, 2019  Dr Sadiye Hayta and Prof Wendy Harwood from the John Innes Centre published their work on an efficient and reproducible Agrobacterium -mediated transformation method for hexaploid wheat, which has important implications for synthetic biology approaches to crop improvements: ... Plant Methods Volume 15, Article number: 121 (2019) https ...

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An efficient and reproducible Agrobacterium-mediated

Oct 26, 2019  DOI: 10.1186/s13007-019-0503-z Corpus ID: 204887129; An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.) @article{Hayta2019AnEA, title={An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.)}, author={Sadiye

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Table 1 from An efficient and reproducible method for

An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201. @article{Wani2011AnEA, title={An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201.}, author ...

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An efficient and reproducible method for development of

An efficient and reproducible method for development of androgenic haploid plants from in vitro anther cultures of Camellia assamica ssp. assamica (Masters) Plant Author:

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PRIME PubMed An efficient and reproducible method for in

An efficient protocol is described for the rapid in vitro clonal propagation of an endangered medicinal plant, Rauvolfia tetraphylla L., through high frequency shoot induction from nodal explants collected from young shoots of a field grown plant. Effects of growth regulators [6-benzyladenine (BA), kinetin (Kin) 2iP, or α-naphthalene acetic acid (NAA)], carbohydrates,

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Efficient and reproducible in vitro regeneration of Solanum ...

Sep 01, 2017  In conclusion, the present study describes an efficient and reproducible method for successful in vitro regeneration of S. lycopersicum by optimizing various parameters, which revealed a significant advantage over the previous methods of in vitro culture of this valuable crop plants, and for the first time, the in vitro regenerated plants were ...

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AN EFFICIENT AND REPRODUCIBLE TISSUE CULTURE

conventional plant breeding (Taji et al., 2002). But an efficient tissue culture protocol is pre-requisite for successful genetic transformation. Therefore, we planned this study to optimize an efficient and reproducible tissue culture procedure for callus induction, multiple shoot regeneration and in vitro root induction in groundnut.

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An efficient and highly reproducible approach for the selection

An efficient and highly reproducible approach for the selection of upland transgenic cotton produced by pollen tube pathway method ... Yuan Y, Sun H (1992) A study of exogenous DNA method of assaying plants transformed with hygromycin or introduction into cultivated soybean. Adv Mol Breed Res PPT resistance genes. Plant Mol Biol Rep. 15: 209 ...

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Efficient and reproducible in vitro regeneration of ... - Europe PMC

Mar 16, 2017  In the present study, we develop an efficient and reproducible in vitro regeneration system for two cultivars viz., Jamila and Tomaland of Solanum lycopersicum L., an economically important vegetable crop throughout the world. Sterilization of seeds with 2.5% (v/v) NaOCl was found to be most effective, about 97% of seeds germinated on cotton in magenta

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An efficient and reproducible method for regeneration of whole

An efficient and reproducible method for regeneration of whole RESEARCH PAPER An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201 Shabir H. Wani1,4,, Gulzar S. Sanghera2 and Satbir S. Gosal3 1 Q2 2 Department of Plant Breeding and Genetics, Punjab Agricultural

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An efficient and reproducible method for regeneration of whole plants

An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201 Author: Shabir H. Wani, Gulzar S. Sanghera, Satbir S. Gosal Source: New biotechnology

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An efficient and reproducible method for regeneration of whole plants

Feb 23, 2011  The highest frequency of shoot regeneration (42.5%) was observed on the MS medium supplemented with 2.0 mg/l BAP, 0.5 mg/l NAA and 0.5 mg/l Kin. The plantlets were hardened and transferred to soil in earthen pots. The developed method was highly reproducible. The in vitro developed plants showed normal growth and flowering under

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An efficient and reproducible method for regeneration of whole plants

An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201 ... In this report we established a reproducible plant regeneration system through somatic embryogenesis. The explants used for regeneration were embryogenic calli derived from mature seeds ...

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PRIME PubMed An efficient and reproducible method for

The highest frequency of shoot regeneration (42.5%) was observed on the MS medium supplemented with 2.0 mg/l BAP, 0.5 mg/l NAA and 0.5 mg/l Kin. The plantlets were hardened and transferred to soil in earthen pots. The developed method was highly reproducible. The in vitro developed plants showed normal growth and flowering under glasshouse ...

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IJPBS Article- AN EFFICIENT AND REPRODUCIBLE METHOD FOR

AN EFFICIENT AND REPRODUCIBLE METHOD FOR IN VITRO PROPAGATION OF CASSIA ALATA L. – AN IMPORTANT WOODY MEDICINAL PLANT MD RAFIQUE AHMED, MOHAMMAD ANIS AND IM AREF: DOI: Abstract: A rapid and efficient protocol for the large-scale propagation of an important woody medicinal plant, Cassia alata L., is described.

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(Free PDF) An efficient and reproducible indirect shoot

A reproducible and more efficient method for indirect shoot organogenesis from female leaf explants has been stan- Keywords 2,4-D . Callus . Differentiation . ... Plant regeneration through tissue cul- ture is a prerequisite tool of biotechnology for successful estab- Culture media and growth conditions lishment of this crop (Kumar et al. 2012

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An efficient and reproducible method for transformation of

The method is based on electroporation of bifidobacterial cells, which were made competent by an optimized methodology based on varying media and growth conditions. Furthermore, the transformation protocol was applied in order to design a PRL2010-derivative, which carries antibiotic resistance against chloramphenicol and which was used to ...

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An efficient and reproducible method for development of

An efficient and reproducible method for development of androgenic haploid plants from in vitro anther cultures of Camellia assamica ssp. assamica (Masters) Plant Author:

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PRIME PubMed An efficient and reproducible method for in

An efficient protocol is described for the rapid in vitro clonal propagation of an endangered medicinal plant, Rauvolfia tetraphylla L., through high frequency shoot induction from nodal explants collected from young shoots of a field grown plant. Effects of growth regulators [6-benzyladenine (BA), kinetin (Kin) 2iP, or α-naphthalene acetic acid (NAA)], carbohydrates,

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Efficient and reproducible somatic embryogenesis and ... - PLOS

May 22, 2019  A dual in vitro regeneration system consisting of indirect organogenesis and somatic embryogenesis (SE), applicable to several varieties of tomato—Solanum lycopersicum (cv. Riogrande, cv. Roma, hybrid 17905 and model cv. M82) has been established. This system is both improved and highly reproducible compared to current methods. Callus initiation, plant

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efficient and reproducible method for transformation of

Aug 01, 2012  Abstract. This study describes an efficient transformation system for the introduction of plasmid DNA into Bifidobacterium bifidum PRL2010 and Bifidobacterium asteroides PRL2011, for which to the best of our knowledge no transformation data have been reported previously. The method is based on electroporation of bifidobacterial cells, which were made

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A Highly Efficient and Reproducible Fusarium spp. Inoculation Method ...

We believe this method will help further develop Brachypodium as a model for ... Heading for disaster: Fusarium graminearum on cereal crops. Mol Plant Pathol 5:515–525 Desjardins AE, Proctor RH (2007) Molecular biology of Fusarium mycotoxins. ... mutant lines). Here, we provide an efficient and reproducible Fusarium infection assay for ...

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Efficient and reproducible in vitro regeneration of ... - Europe PMC

Mar 16, 2017  In the present study, we develop an efficient and reproducible in vitro regeneration system for two cultivars viz., Jamila and Tomaland of Solanum lycopersicum L., an economically important vegetable crop throughout the world. Sterilization of seeds with 2.5% (v/v) NaOCl was found to be most effective, about 97% of seeds germinated on cotton in magenta

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